THE JOURNAL OF VITAMINGLOGY 16, 293-296 1970) Determination of Vitamins D by Gas-Liquid Chromatography

The gas chromatographic conditions for the assay of vitamins D were described. The analysis was performed on Shimazu Gas Chromatograph GC 4AP equipped with a hydrogen flame ionization detector. Glass columns (4 i.d.•~ 1000mm) packed with 1.5% OV-17 on Shimalite W, 80-100 mesh, silanized, were operated at 240•‹ and a flow rate of 40ml/min (N2) was maintained. Adoption of "unmodified" D as an analytical form and good GLC resolution under the operated condition made the procedure more simple and time-saving. Employment of 7-dehydrocholestery) acetate as an internal standard enhanced the accuracy of the analysis. Satisfactory result was obtained in the determina tion of vitamins D2 and D3 in mixtures.